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Antibodies bound to the blocking peptide no more bind to the epitope to the target protein. By comparing the staining through the blocked antibody compared to the antibody by itself, you can see which staining is particular.

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This guideline outlines additional feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

This guidebook outlines more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...

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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...

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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide usually is made up of the epitope identified by the antibody.

Target Data Cyclin E belongs to your really conserved cyclin family members, whose members are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation designs which contribute towards the temporal coordination of each mitotic party. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress through S period.

Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and therefore may lead to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical part in promoting cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two added splice variants ended up reported but comprehensive nucleotide sequence info just isn't still out there.

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